About   Help   FAQ
Leprtm1Yli
Targeted Allele Detail
Summary
Symbol: Leprtm1Yli
Name: leptin receptor; targeted mutation 1, Yong Liu
MGI ID: MGI:3822311
Synonyms: Ob-RbF985/1077/1138, Y123F
Gene: Lepr  Location: Chr4:101574601-101672549 bp, + strand  Genetic Position: Chr4, 46.96 cM
Alliance: Leprtm1Yli page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:142218
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129
Mutation
description
Allele Type:    Targeted
Mutations:    Insertion, Nucleotide substitutions
 
Mutation detailsThree tyrosine residues in exon 18 (985, 1077, 1138) were replaced with phenylalanine and a floxed neo cassette was inserted after exon 18 via homologous recombination. The floxed neo cassette was then removed by cre mediated recombination leaving behind a single loxP site. Quantitative RT-PCR indicated that expression of the long isoform in the hypothalamus of homozygotes was similar to that in wild-type mice. Replacement of these tyrosines with phenylalanines abolished leptin stimulated STAT3 phosphorylation in the hypothalamus. (J:142218)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Loading...
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Lepr Mutation:  122 strains or lines available
References
Original:  J:142218 Jiang L, et al., Tyrosine-dependent and -independent actions of leptin receptor in control of energy balance and glucose homeostasis. Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18619-24
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
12/17/2024
MGI 6.24
The Jackson Laboratory