Leprtm1Yli
Targeted Allele Detail
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| Symbol: |
Leprtm1Yli |
| Name: |
leptin receptor; targeted mutation 1, Yong Liu |
| MGI ID: |
MGI:3822311 |
| Synonyms: |
Ob-RbF985/1077/1138, Y123F |
| Gene: |
Lepr Location: Chr4:101574601-101672549 bp, + strand Genetic Position: Chr4, 46.96 cM
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| Alliance: |
Leprtm1Yli page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:142218
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
129
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| Allele Type: |
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Targeted |
| Mutations: |
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Insertion, Nucleotide substitutions
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Mutation details: Three tyrosine residues in exon 18 (985, 1077, 1138) were replaced with phenylalanine and a floxed neo cassette was inserted after exon 18 via homologous recombination. The floxed neo cassette was then removed by cre mediated recombination leaving behind a single loxP site. Quantitative RT-PCR indicated that expression of the long isoform in the hypothalamus of homozygotes was similar to that in wild-type mice. Replacement of these tyrosines with phenylalanines abolished leptin stimulated STAT3 phosphorylation in the hypothalamus.
(J:142218)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Lepr Mutation: |
122 strains or lines available
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| Original: |
J:142218 Jiang L, et al., Tyrosine-dependent and -independent actions of leptin receptor in control of energy balance and glucose homeostasis. Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18619-24 |
| All: |
3 reference(s) |
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Analysis Tools
